Safe handling of specimen container in the laboratory

Specimen container

1. The container for infectious specimens can be glass, but in order to ensure safety, it is best to use plastic products (depending on actual needs).

2. The container of the specimen should be sturdy and not easily broken. There should be no liquid leakage after the lid or stopper is closed. All specimens should be stored in containers.

3. The container should be properly labeled for identification, and the label should have the necessary information such as sample name, date of collection, and number.

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The sample form and / or instructions should not be tied outside the container, but should be placed in a waterproof bag separately to prevent contamination and affect the use.

Receipt of specimens

1. Laboratories that need to receive a large number of samples should designate special rooms or spaces.

2. Personnel receiving and unpacking samples should be aware of the potential health hazards of the samples and trained in the use of routine precautions, especially when handling broken or leaking containers.

3. The sample should be opened in the biosafety cabinet and prepared with disinfectant. Operators should wear waterproof protective clothing, biological safety masks, eye masks, and gloves.

4. Before opening the package, you should carefully check the appearance and label of each container, whether the label and inspection report are consistent with the contents, whether there is contamination and whether the container is damaged, etc., you must register a detailed report and record the disposal method. .

5. The samples should not be received and opened in the office and ordinary BSL-1 laboratory.

Opening of specimen tubes or specimen containers

1. The sample tube should be opened in a Class I or II biosafety cabinet.

2. Gloves must be worn. Eye and mucous membrane protection [goggles or face shield (helmet)] is recommended.

3. A plastic apron should be worn outside the protective clothing.

4. When opening the stopper, wrap the stopper with paper or gauze to prevent splashing.

Handling of films and smears for microscopy

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   Blood, saliva, and stool samples used for microscopic observation do not have to kill all microorganisms and viruses on the smear when they are fixed and stained, so these things should be taken with tweezers, stored properly, and decontaminated and (or ) Discard after autoclaving.

Handling of tissue specimens

Tissue samples should be fixed in formalin. Small samples, such as needle biopsy samples, can be fixed and sterilized within hours, but larger samples can take days.

Avoid frozen sections. If necessary, cover the freezer and wear a protective face shield. During disinfection, the temperature of the instrument should be raised to at least 20 ° C.

Decontamination

  Hypochlorite and high-level disinfectants are recommended to remove contamination. Generally, a freshly prepared hypochlorite solution containing 1 g / L of available chlorine can be used. When treating spilled blood, the effective chlorine concentration should reach 10 g / L. Glutaraldehyde can be used to remove surface contamination.

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